C3 / C3b antibody [C3], C-term
Catalogue Number: GTX101316-GTX
GTX101316 Image
GTX101316 Image
GTX101316 Image
GTX101316 Image
GTX101316 Image
GTX101316 Image
Immunoprecipitation of C3 protein from HepG2 whole cell extracts using 5 µg of C3 antibody [C3], C-term (GTX101316). Western blot analysis was performed using C3 antibody [C3], C-term (GTX101316). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
GTX101316 Image
C3 antibody [C3], C-term detects C3 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: C3 protein stained by C3 antibody [C3], C-term (GTX101316) diluted at 1:200. Blue: Hoechst 33342 staining.
GTX101316 Image
C3 antibody [C3], C-term detects C3 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. C3 antibody [C3], C-term (GTX101316) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with C3 antibody (GTX101316) diluted at 1:1000.
GTX101316 Image
Human plasma (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with C3 / C3b antibody [C3], C-term (GTX101316) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 187 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | complement C3 , AHUS5 , ARMD9 , ASP , C3a , C3b , CPAMD1 , HEL-S-62p |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the C-terminus region of human C3. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, IP, WB, ImmSub |
Description
Description: Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. People with C3 deficiency are susceptible to bacterial infection. [provided by RefSeq]
Additional Text
Gene Name
C3
Uniprot ID
P01024
Gene ID
718
Purification
Affinity Purified
Molecular Weight
187
Concentration
0.07 mg/ml
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX101316 Datasheet
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