ACE2 antibody [N1N2], N-term
Catalogue Number: GTX101395-GTX
GTX101395 Image
GTX101395 Image
GTX101395 Image
GTX101395 Image
GTX101395 Image
GTX101395 Image
GTX101395 Image
GTX101395 Image
GTX101395 Image
GTX101395 Image
GTX101395 Image
GTX101395 Image
GTX101395 Image
GTX101395 Image
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Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX135683-pro Human ACE2 (ECD) protein , mouse IgG Fc tag protein (1 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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ACE2 antibody [N1N2], N-term detects ACE2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human lung. ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX101395 Image
ACE2 antibody [N1N2], N-term detects ACE2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX101395 Image
ACE2 antibody [N1N2], N-term detects ACE2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat lung. ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX101395 Image
ACE2 antibody [N1N2], N-term detects ACE2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Indirect ELISA analysis performed by coating plate with recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag protein (GTX135683-pro) (15.63-1000 ng/mL). Coated protein probed with ACE2 antibody [N1N2], N-term (GTX101395) (0.1 µg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.
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Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (coated at 2 µg/mL) binding to soluble recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag (GTX135683-pro) (4.5-10000 ng/mL). Bound protein was detected by ACE2 antibody [N1N2], N-term (GTX101395) (0.5 µg/mL) and Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.
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ACE2 antibody [N1N2], N-term detects ACE2 protein at cell membrane by immunofluorescent analysis. Sample: Vero E6 cells were fixed in ice-cold MeOH for 5 min. Green: ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).
GTX101395 Image
Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX101395 Image
ACE2 antibody [N1N2], N-term detects ACE2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX101395 Image
ACE2 antibody [N1N2], N-term detects ACE2 protein at cell membrane by immunofluorescent analysis. Sample: Vero E6 cells were fixed in ice-cold MeOH for 5 min. Green: ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10 µm.
GTX101395 Image
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX101395 Image
Untreated (-) and treated (+) Vero E6 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX101395 Image
Non-transfected (-) and transfected (+) Caco-2 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin™ 300 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | angiotensin I converting enzyme 2 , ACEH |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul, 10 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the N-terminus region of human ACE2. The exact sequence is proprietary. |
| Application: | ELISA, FACS, ICC, IF, IHC-P, WB |
Description
Description: The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq]
Additional Text
Uniprot ID
Q9BYF1
Gene Name
ACE2
Gene ID
59272
Concentration
0.2 mg/ml
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Gene Alias
angiotensin I converting enzyme 2 , ACEH
Molecular Weight
92
GTX101395 Datasheet
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