ALDH2 antibody
Catalogue Number: GTX101429-GTX
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ELISA detection of ALDH2 using GTX101429 for capture at a concentration of 2.5 µg/mL and GTX88073 for detection at a concentration of 1.5 µg/mL.
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Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using ALDH2 antibody (GTX101429) at 1:300 dilution.
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ALDH2 antibody detects ALDH2 protein at mitochondria on whole-mount zebrafish embryos by immunohistochemical analysis. Sample: Paraformaldehyde-fixed zebrafish embryos. ALDH2 antibody (GTX101429) dilution: 1:200.
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ALDH2 antibody detects ALDH2 protein by western blot analysis. A. 30 µg GL261 whole cell lysate/extract B. 30 µg C8D30 whole cell lysate/extract C. 30 µg BCL-1 whole cell lysae/extract D. 30 µg Raw264.7 whole cell lysate/extract 7.5% SDS-PAGE ALDH2 antibody (GTX101429) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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ALDH2 antibody detects ALDH2 protein by Western blot analysis. Whole cell extracts (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH2 antibody (GTX101429) at a dilution of 1:500.
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ALDH2 antibody detects ALDH2 protein by Western blot analysis. Zebrafish tissue extracts (30 µg) was separated by 10 % SDS-PAGE, and the membrane was blotted with ALDH2 antibody (GTX101429) at a dilution of 1:2000.
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HepG2 whole cell and cytoplasm extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ALDH2 antibody (GTX101429) diluted at 1:500.
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ALDH2 antibody detects ALDH2 protein at mitochondria on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. ALDH2 antibody (GTX101429) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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ALDH2 antibody detects ALDH2 protein at mitochondria on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. ALDH2 antibody (GTX101429) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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Immunohistochemical analysis of paraffin-embedded AGS xenograft, using ALDH2(GTX101429) antibody at 1:100 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 56 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | aldehyde dehydrogenase 2 family member , ALDH-E2 , ALDHI , ALDM |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human ALDH2. The exact sequence is proprietary. |
| Application: | ELISA, IHC-P, sELISA, WB, IHC, IHC-WM |
Description
Description: This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. [provided by RefSeq]
Additional Text
Gene Name
ALDH2
Gene ID
217
Uniprot ID
P05091
Concentration
0.23 mg/ml
Purification
Affinity Purified
Molecular Weight
56
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:3000. IHC-P: 1:100-1:1000. ELISA: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX101429 Datasheet
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DELIVERY
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