beta Catenin antibody [N1N2-2], N-term
Catalogue Number: GTX101435-GTX
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Cross-linked ChIP was performed with HCT116 chromatin extract and 5 µg of either control rabbit IgG or anti-beta Catenin antibody. The precipitated DNA was detected by PCR with primer set targeting to c-Myc promoter.
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Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using beta Catenin(GTX101435) antibody (Green) at 1:200 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
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Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using beta Catenin antibody [N1N2-2], N-term (GTX101435) at 1:300 dilution.
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Immunohistochemical analysis of agarose-embedded zebrafish embryo, using beta Catenin antibody [N1N2-2], N-term GTX101435) at 1:100. dilution. (This image was provided courtesy of the Schilling Lab at UC, Irvine.)
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Sample (50 µg of whole cell lysate) A: mouse brain 7.5% SDS PAGE GTX101435 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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beta Catenin antibody [N1N2-2], N-term detects CTNNB1 protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract | 7.5% SDS-PAGE beta Catenin antibody [N1N2-2], N-term (GTX101435) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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beta Catenin antibody [N1N2-2], N-term detects Ctnnb1 protein on zebrafish by whole mount immunohistochemical analysis. Sample: 1 day-post-fertilization zebrafish embryo. beta Catenin antibody [N1N2-2], N-term (GTX101435) dilution: 1:100.
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beta Catenin antibody [N1N2-2] detects beta Catenin protein at cell membrane in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Green: beta Catenin antibody [N1N2-2] (GTX101435) diluted at 1:500. Red: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500. Blue: Hoechst 33342 staining. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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beta Catenin antibody [N1N2-2] detects beta Catenin protein at cell membrane in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Green: beta Catenin antibody [N1N2-2] (GTX101435) diluted at 1:500. Red: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500. Blue: Hoechst 33342 staining. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Immunoprecipitation of beta Catenin protein from HeLa whole cell extracts using 5 µg of beta Catenin antibody (GTX101435). Western blot analysis was performed using beta Catenin antibody (GTX101435). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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beta Catenin antibody [N1N2-2], N-term (GTX101435) detects CTNNB1 protein by flow cytometry analysis. Sample: HeLa cell. Black: Unlabelled sample was used as a control. Red: beta Catenin antibody [N1N2-2], N-term (GTX101435) dilution: 1:50. Acquisition of 20,000 events were collected for FACS analysis.
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beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in human esophagus by immunohistochemical analysis. Sample: Paraffin-embedded human esophagus. beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in human cervix by immunohistochemical analysis. Sample: Paraffin-embedded human cervix. beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: beta Catenin protein stained by beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500. Blue: Hoechst 33342 staining.
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beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody (GTX101435) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody (GTX101435) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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beta Catenin antibody detects beta Catenin protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded cat liver. beta Catenin stained by beta Catenin antibody (GTX101435) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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beta Catenin antibody detects beta Catenin protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded cat colon. beta Catenin stained by beta Catenin antibody (GTX101435) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membranes were blotted with beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:1000 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.
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beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Wild-type (WT) and beta Catenin knockout (KO) 293T cell extracts (9 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Various tissue extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody (GTX101435) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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beta Catenin antibody [N1N2-2], N-term detects Ctnnb1 protein on zebrafish by whole mount immunohistochemical analysis. Sample: 2 days-post-fertilization zebrafish embryo. beta Catenin antibody [N1N2-2], N-term (GTX101435) dilution: 1:100.
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beta Catenin antibody [N1N2-2], N-term detects Ctnnb1 protein on zebrafish by whole mount immunohistochemical analysis. Sample: 2 days-post-fertilization zebrafish embryo. beta Catenin antibody [N1N2-2], N-term (GTX101435) dilution: 1:100.
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beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in rat colon by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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beta Catenin antibody detects beta Catenin protein at cell membrane by immunofluorescent analysis. Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: beta Catenin stained by beta Catenin antibody (GTX101435) diluted at 1:1000.
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beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in rat duodenum by immunohistochemical analysis. Sample: Paraffin-embedded rat duodenum. beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane by immunofluorescent analysis. Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: beta Catenin protein stained by beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500. Blue: Hoechst 33342 staining.
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beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. beta Catenin stained by beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. beta Catenin stained by beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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The data was published in the journal Evid Based Complement Alternat Med in 2012.PMID: 23320038
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The data was published in the journal Oncotarget in 2018. PMID: 30613366
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The data was published in the journal Cell Adh Migr in 2019. PMID: 30744493
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beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at membrane on mouse urinary bladder by immunohistochemical analysis. Sample: Paraffin-embedded mouse urinary bladder. beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at membrane on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. beta Catenin antibody [N1N2-2], N-term (GTX101435) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at membrane on mouse skin by immunohistochemical analysis. Sample: Paraffin-embedded mouse skin. beta Catenin antibody [N1N2-2], N-term (GTX101435) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:10000.
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 85 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | catenin beta 1 , CTNNB , EVR7 , MRD19 , armadillo |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the N-terminus region of human beta Catenin. The exact sequence is proprietary. |
| Application: | FC, ICC, IF, IHC-P, IP, ChIP, WB, IHC-Fr, IHC, IHC-WM |
Description
Description: Beta-catenin is an adherens junction protein. Adherens junctions (AJs; also called the zonula adherens) are critical for the establishment and maintenance of epithelial layers, such as those lining organ surfaces. AJs mediate adhesion between cells, communicate a signal that neighboring cells are present, and anchor the actin cytoskeleton. In serving these roles, AJs regulate normal cell growth and behavior. At several stages of embryogenesis, wound healing, and tumor cell metastasis, cells form and leave epithelia. This process, which involves the disruption and reestablishment of epithelial cell-cell contacts, may be regulated by the disassembly and assembly of AJs. AJs may also function in the transmission of the 'contact inhibition' signal, which instructs cells to stop dividing once an epithelial sheet is complete.[supplied by OMIM]
Additional Text
Gene Name
CTNNB1
Gene ID
1499
Concentration
0.13 mg/ml
Uniprot ID
P35222
Purification
Affinity Purified
Molecular Weight
85
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Short Description
Beta-catenin antibody recognizes beta-catenin protein, which acts as a cell adhesion factor as well as an effector of Wnt signaling pathway-driven transcription. Mutation or dysregulation of beta-catenin is associated with many diseases, including developmental disorders and cancers. There are a plethora of commercially available beta-catenin antibodies, among which GeneTex's beta-catenin antibody (GTX101435) is one of the most rigorously validated and widely cited for a number of applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Application Notes
WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. FCM: 1:50-1:200. IP: 1:50-1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX101435 Datasheet
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