Iba1 antibody
Catalogue Number: GTX101495-GTX
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Flow cytometry on primary murine microglia cells, staining with Iba1 (GTX101495) antibody using 1.0 µg per 4x105 cells. GTX101495 (blue), Rabbit IgG (green) ,Unstained (red).
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Immunoprecipitation of Iba1 protein from K562 whole cell extracts using 5 µg of Iba1 antibody (GTX101495). Western blot analysis was performed using Iba1 antibody (GTX101495). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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Iba1 antibody (GTX101495) detects AIF1 protein by flow cytometry analysis. Sample: THP-1 cell. Black: Unlabelled sample was used as a control. Red: Iba1 antibody (GTX101495) dilution: 1:50. Acquisition of 20,000 events were collected for FACS analysis.
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Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membranes were blotted with Iba1 antibody (GTX101495) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.
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Various tissue extracts (50 µg) were separated by 15% SDS-PAGE, and the membranes were blotted with Iba1 antibody (GTX101495) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. *The competitor is not affiliated with GeneTex and does not endorse this product.
GTX101495 Image
Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (GTX101495) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. Iba1 stained by Iba1 antibody (GTX101495) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. Iba1 stained by Iba1 antibody (GTX101495) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Iba1 antibody detects Iba1 protein at cell periphery by immunofluorescent analysis. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Iba1 stained by Iba1 antibody (GTX101495) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
GTX101495 Image
Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Iba1 stained by Iba1 antibody (GTX101495) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX101495 Image
Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Iba1 stained by Iba1 antibody (GTX101495) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX101495 Image
Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Iba1 stained by Iba1 antibody (GTX101495) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX101495 Image
Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Iba1 stained by Iba1 antibody (GTX101495) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX101495 Image
Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Iba1 stained by Iba1 antibody (GTX101495) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX101495 Image
Various tissue extracts (50 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (GTX101495) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX101495 Image
Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (GTX101495) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 17 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | allograft inflammatory factor 1 , AIF-1 , IBA1 , IRT-1 , IRT1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human Iba1. The exact sequence is proprietary. |
| Application: | FC, ICC, IF, IHC-P, IP, WB, IHC-Fr |
Description
Description: This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Additional Text
Uniprot ID
P55008
Gene Name
AIF1
Purification
Affinity Purified
Gene ID
199
Concentration
0.14 mg/ml
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
17
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Application Notes
WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. FCM: 1:50-1:200. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX101495 Datasheet
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