NSE antibody [N1C1]
Catalogue Number: GTX101553-GTX
GTX101553 Image
GTX101553 Image
GTX101553 Image
GTX101553 Image
GTX101553 Image
GTX101553 Image
GTX101553 Image
GTX101553 Image
NSE antibody [N1C1] detects NSE protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: NSE protein stained by NSE antibody [N1C1] (GTX101553) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).
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Sample (50 µg of whole cell lysate) A: mouse brain 10% SDS PAGE GTX101553 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX101553 Image
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with NSE antibody [N1C1] (GTX101553) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX101553 Image
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with NSE antibody [N1C1] (GTX101553) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX101553 Image
The data was published in the journal Oncotarget in 2016.PMID: 27034167
GTX101553 Image
The data was published in the journal Oncotarget in 2016.PMID: 27034167
GTX101553 Image
Immunohistochemical analysis of paraffin-embedded PC14 xenograft, using ENO2(GTX101553) antibody at 1:100 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 47 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | enolase 2 , HEL-S-279 , NSE |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human NSE. The exact sequence is proprietary. |
| Application: | IHC-P, WB, IHC-Fr |
Description
Description: This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq]
Additional Text
Gene Name
ENO2
Gene ID
2026
Uniprot ID
P09104
Concentration
0.13 mg/ml
Purification
Affinity Purified
Molecular Weight
47
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:1000-1:10000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Short Description
NSE is a 78 kDa gamma-homodimer expressed as a dominant enolase-isoenzyme in neuronal and neuroendocrine tissues. Elevated levels of serum NSE are often associated with neuronal disorders and neural crest-derived tumors. Up to 70 percent of patients with small cell lung carcinoma (SCLC) have high levels of serum NSE, as SCLC cells can express neuronal gene programs.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX101553 Datasheet
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