GAD67 antibody
Catalogue Number: GTX101881-GTX
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GAD67 antibody detects GAD67 protein on myofiber membranes by immunohistochemical analysis. Sample: Agarose-embedded zebrafish embryo GAD67 antibody (GTX101881) dilution: 1:200. Image provided with permission courtesy of Dr. T. Schilling at UC, Irvine.
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GAD67 antibody detects GAD67 protein on myofiber membranes by immunohistochemical analysis. Sample: Whole-mount zebrafish embryo GAD67 antibody (GTX101881) dilution: 1:200.
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GAD67 antibody detects GAD67 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg whole cell lysate/extract of human GAD1-transfected 293T cells 7.5% SDS-PAGE GAD67 antibody (GTX101881) dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 antibody (GTX101881) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GAD67 antibody detects GAD67 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GAD67 stained by GAD67 antibody (GTX101881) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
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Various tissue extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 antibody (GTX101881) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX101881 Image
Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 antibody (GTX101881) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Human tissue extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 antibody (GTX101881) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GAD67 antibody detects GAD67 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GAD67 stained by GAD67 antibody (GTX101881) diluted at 1:500. Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
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GAD67 antibody detects GAD67 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. GAD67 stained by GAD67 antibody (GTX101881) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GAD67 antibody detects GAD67 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. GAD67 stained by GAD67 antibody (GTX101881) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX101881 Image
GAD67 antibody detects GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. GAD67 stained by GAD67 antibody (GTX101881) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX101881 Image
GAD67 antibody detects GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. GAD67 stained by GAD67 antibody (GTX101881) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX101881 Image
GAD67 antibody detects GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse eye. Green: GAD67 stained by GAD67 antibody (GTX101881) diluted at 1:250. Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 67 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | glutamate decarboxylase 1 , CPSQ1 , GAD , SCP |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the N-terminus region of human GAD67. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB, IHC-Fr, IHC, IHC-WM, IHC-Flt |
Description
Description: This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq]
Additional Text
Gene Name
GAD1
Gene ID
2571
Uniprot ID
Q99259
Concentration
0.22 mg/ml
Purification
Affinity Purified
Molecular Weight
67
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX101881 Datasheet
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