GOT2 antibody
Catalogue Number: GTX101929-GTX
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GTX101929 Image
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GOT2 antibody detects GOT2 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. GOT2 antibody (GTX101929) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GOT2 antibody detects GOT2 protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. GOT2 antibody (GTX101929) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GOT2 antibody (GTX101929) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Immunofluorescence analysis of methanol-fixed HeLa, using GOT2(GTX101929) antibody at 1:500 dilution.
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GOT2 antibody detects GOT2 protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract 10% SDS-PAGE GOT2 antibody (GTX101929) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GOT2 antibody detects GOT2 protein by western blot analysis. A. 50 µg rat lung lysate/extract 10% SDS-PAGE GOT2 antibody (GTX101929) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Sample (30 µg of whole cell lysate) A: HepG2 10% SDS PAGE GTX101929 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using GOT2(GTX101929) antibody at 1:500 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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GOT2 antibody detects GOT2 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma tissue. GOT2 antibody (GTX101929) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Thimerosal|0.01% Thimerosal |
| Molecular Weight: | 48 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | glutamic-oxaloacetic transaminase 2 , KAT4 , KATIV , KYAT4 , mitAAT |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human GOT2. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB |
Description
Description: Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq]
Additional Text
Gene Name
GOT2
Uniprot ID
P00505
Purification
Affinity Purified
Gene ID
2806
Molecular Weight
48
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX101929 Datasheet
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