KAP1 antibody [N3C2], Internal
Catalogue Number: GTX102226-GTX
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KAP1 antibody immunoprecipitates KAP1 protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 50 µg HeLa whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of KAP1 protein by 2 µg of KAP1 antibody (GTX102226) 7.5% SDS-PAGE The immunoprecipitated KAP1 protein was detected by KAP1 antibody (GTX102226) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
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KAP1 antibody [N3C2], Internal detects KAP1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: KAP1 stained by KAP1 antibody [N3C2], Internal (GTX102226) diluted at 1:2000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 µm.
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Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with KAP1 antibody [N3C2], Internal (GTX102226) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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KAP1 antibody [N3C2], Internal detects KAP1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. KAP1 stained by KAP1 antibody [N3C2], Internal (GTX102226) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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KAP1 antibody [N3C2], Internal detects KAP1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. KAP1 stained by KAP1 antibody [N3C2], Internal (GTX102226) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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ChIP assay followed by QPCR on a known KAP1-binding region within the ZNF180 3'UTR (Mol Cell Biol, 2011, Lyengar et al)
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Sample (30 µg of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE GTX102226 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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The data was published in the journal Nutrients in 2018.PMID: 30200495
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Immunohistochemical analysis of paraffin-embedded human breast cancer, using KAP1(GTX102226) antibody at 1:500 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 89 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | tripartite motif containing 28 , KAP1 , PPP1R157 , RNF96 , TF1B , TIF1B |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human KAP1. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, IP, ChIP, WB |
Description
Description: The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq]
Additional Text
Gene Name
TRIM28
Concentration
0.48 mg/ml
Purification
Affinity Purified
Gene ID
10155
Molecular Weight
89
Uniprot ID
Q13263
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:500-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX102226 Datasheet
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