p63 antibody [N2C1], Internal
Catalogue Number: GTX102425-GTX
GTX102425 Image
GTX102425 Image
GTX102425 Image
GTX102425 Image
GTX102425 Image
GTX102425 Image
GTX102425 Image
GTX102425 Image
GTX102425 Image
GTX102425 Image
GTX102425 Image
GTX102425 Image
Immunoprecipitation of p63 protein from A431 whole cell extracts using 5 µg of p63 antibody [N2C1], Internal (GTX102425). Western blot analysis was performed using p63 antibody [N2C1], Internal (GTX102425). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
GTX102425 Image
p63 antibody [N2C1], Internal detects TP63 protein by western blot analysis. A. 50 µg mouse brain lysate/extract 7.5% SDS-PAGE p63 antibody [N2C1], Internal (GTX102425) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX102425 Image
p63 antibody [N2C1], Internal detects TP63 protein by western blot analysis. A. 50 µg rat brain lysate/extract 7.5% SDS-PAGE p63 antibody [N2C1], Internal (GTX102425) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX102425 Image
Non-transfected (-) and transfected (+) A431 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p63 antibody [N2C1], Internal (GTX102425) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX102425 Image
p63 antibody [N2C1], Internal detects p63 protein at nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: p63 stained by p63 antibody [N2C1], Internal (GTX102425) diluted at 1:500. Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
GTX102425 Image
p63 antibody [N2C1], Internal detects p63 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse tongue. p63 stained by p63 antibody [N2C1], Internal (GTX102425) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX102425 Image
The data was published in the journal PLoS Pathog in 2018. PMID: 30543708
GTX102425 Image
The data was published in the journal Sci Rep in 2018.PMID: 30120338
GTX102425 Image
Immunohistochemical analysis of paraffin-embedded SCC4 xenograft, using p63(GTX102425) antibody at 1:100 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
GTX102425 Image
Immunohistochemical characterization of Synaptophysin (GTX100865), p63 (GTX102425) and Cytokeratin 7 (GTX109723) in human small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC) specimens. Sample: Paraffin-embedded human SCLC (upper panel) and NSCLC (lower panel). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 15 mins. The section was then incubated with primary antibody at 1:500 overnight at 4oC and detected using an HRP conjugated avidin-biotin-peroxidase Complex system. DAB was used as the chromogen and counterstained with haematoxylin. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX102425 Image
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p63 antibody [N2C1], Internal (GTX102425) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 77 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | tumor protein p63 , AIS , B(p51A) , B(p51B) , EEC3 , KET , LMS , NBP , OFC8 , RHS , SHFM4 , TP53CP , TP53L , TP73L , p40 , p51 , p53CP , p63 , p73H , p73L |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human p63. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, IP, WB, IHC-Fr, PLA |
Description
Description: This gene encodes a member of the p53 family of transcription factors. An animal model, p63 -/- mice, has been useful in defining the role this protein plays in the development and maintenance of stratified epithelial tissues. p63 -/- mice have several developmental defects which include the lack of limbs and other tissues, such as teeth and mammary glands, which develop as a result of interactions between mesenchyme and epithelium. Mutations in this gene are associated with ectodermal dysplasia, and cleft lip/palate syndrome 3 (EEC3); split-hand/foot malformation 4 (SHFM4); ankyloblepharon-ectodermal defects-cleft lip/palate; ADULT syndrome (acro-dermato-ungual-lacrimal-tooth); limb-mammary syndrome; Rap-Hodgkin syndrome (RHS); and orofacial cleft 8. Both alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different proteins. Many transcripts encoding different proteins have been reported but the biological validity and the full-length nature of these variants have not been determined. [provided by RefSeq]
Additional Text
Gene Name
TP63
Gene ID
8626
Uniprot ID
Q9H3D4
Purification
Affinity Purified
Molecular Weight
77
Concentration
1.37 mg/ml
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX102425 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations