Catalogue Number: GTX102474-GTX
Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with TMS1 antibody [N1C3] (GTX102474) diluted at 1:500.
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with TMS1 antibody [N1C3] (GTX102474) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
TMS1 antibody [N1C3] detects TMS1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HCT 116 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: TMS1 protein stained by TMS1 antibody [N1C3] (GTX102474) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
|Type:||Polyclonal Primary Antibody - Unconjugated|
|Alias:||PYD and CARD domain containing , ASC , CARD5 , TMS , TMS-1 , TMS1|
|Shipping Condition:||Blue Ice|
|Unit(s):||100 ul, 25 ul|
|Immunogen:||Recombinant protein encompassing a sequence within the center region of human TMS1. The exact sequence is proprietary.|
|Application:||ICC, IF, WB, IHC-Fr|
Description: This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-Fr: Assay dependent. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption