ACAT1 antibody [N1N3]
Catalogue Number: GTX102637-GTX
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GTX102637 Image
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ACAT1 antibody [N1N3] detects ACAT1 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ACAT1 protein stained by ACAT1 antibody [N1N3] (GTX102637) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000. Blue: Hoechst 33342 staining.
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Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE GTX102637 diluted at 1:1000
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Sample (30 µg of whole cell lysate) A: whole zebrafish 10% SDS PAGE GTX102637 diluted at 1:1000
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Sample(30 µg of whole cell lysate) A:293T B:A431(GTX27909) C:MOLT4(GTX27912) 10% SDS PAGE GTX102637 diluted at 1:500
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ACAT1 antibody [N1N3] detects ACAT1 protein at cytoplasm on human normal kidney by immunohistochemical analysis. Sample: Paraffin-embedded human normal kidney. ACAT1 antibody [N1N3] (GTX102637) diluted at 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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ACAT1 antibody [N1N3] detects ACAT1 protein at mitochondria by immunofluorescent analysis. Sample: A431 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: ACAT1 protein stained by ACAT1 antibody [N1N3] (GTX102637) diluted at 1:500. Blue: Hoechst 33342 staining.
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Thimerosal|0.01% Thimerosal |
| Molecular Weight: | 45 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | acetyl-CoA acetyltransferase 1 , ACAT , MAT , T2 , THIL |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the N-terminus region of human ACAT1. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB |
Description
Description: This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq]
Additional Text
Gene ID
38
Gene Name
ACAT1
Uniprot ID
P24752
Purification
Affinity Purified
Molecular Weight
45
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX102637 Datasheet
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