alpha Actinin 3 antibody
Catalogue Number: GTX103216-GTX
GTX103216 Image
GTX103216 Image
GTX103216 Image
GTX103216 Image
GTX103216 Image
GTX103216 Image
alpha Actinin 3 antibody detects alpha Actinin 3 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 3 antibody (GTX103216) diluted by 1:10000.
GTX103216 Image
Immunoprecipitation of alpha Actinin 3 protein from HeLa whole cell extracts using 5 µg of alpha Actinin 3 antibody (GTX103216). Western blot analysis was performed using alpha Actinin 3 antibody (GTX103216). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7.5% SDS PAGE GTX103216 diluted at 1:10000
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Sample (30 ug of whole cell lysate) A: PC-12 7.5% SDS PAGE GTX103216 diluted at 1:10000
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Sample (30 µg of whole cell lysate) A: whole zebrafish 7.5% SDS PAGE GTX103216 diluted at 1:1000
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Thimerosal|0.01% Thimerosal |
| Molecular Weight: | 103 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | actinin alpha 3 (gene/pseudogene) , ACTN3D |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human alpha Actinin 3. The exact sequence is proprietary. |
| Application: | IP, WB |
Description
Description: Alpha-actinin is an actin-binding protein with multiple roles in different cell types. This gene expression is limited to skeletal muscle. It is localized to the Z-disc and analogous dense bodies, where it helps to anchor the myofibrillar actin filaments [provided by RefSeq]
Additional Text
Gene ID
89
Gene Name
ACTN3
Uniprot ID
Q08043
Purification
Affinity Purified
Molecular Weight
103
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:5000-1:20000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX103216 Datasheet
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