CCL21 antibody
Catalogue Number: GTX10364-GTX
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No preservative |
| Molecular Weight: | 15 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | chemokine (C-C motif) ligand 21A (serine) , 6CKBAC2 , 6Ckine , ALP , AW987545 , CKb9 , SCYA21a , SLC , Scya21 , Scya21b , Tca4 , plt |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ug |
| Host name: | Goat |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | recombinant mouse 6Ckine, expressed in Escherichia coli. |
| Application: | ELISA, WB, NT, Inh |
Description
Description: Mouse 6Ckine is a beta- or C-C chemokine identified in the Expressed Sequence Tag (EST) database by three independent groups (Hedrick and Zlotnik, Hromas et al, Nagira et al). Known also as Exodus-2 and secondary lymphoid-tissue chemokine (SLC), 6Ckine contains four conserved cysteine (C) residues which are characteristic of beta-chemokines (Hedrick and Zlotnik, Hromas et al, Nagira et al). Two additional conserved cysteine residues have been found in its unusually long carboxy-terminal domain and consequently the name 6Ckine (Hedrick and Zlotnik, Hromas et al, Nagira et al). Human and mouse 6Ckine are highly conserved and show 86% amino acid homology.1 Mouse 6Ckine cDNA encodes a 133 amino acid precursor protein, a 23 amino acid signal peptide and a 110 amino acid mature protein. (Hedrick and Zlotnik). Comparatively, human 6Ckine cDNA encodes a 134 amino acid precursor protein, a 23 amino acid signal peptide and a 111 amino acid mature protein(Hedrick and Zlotnik, Nagira et al). Recombinant mouse 6Ckine has a predicted molecular mass of approximately 12 kDa (mature protein). In SDS-PAGE under reducing and non-reducing conditions, the recombinant protein migrates with an apparent molecular mass of 16 kDa to 17 kDA.The expression of mouse 6Ckine has been detected in high levels in the spleen and lung (Nagira et al). Recombinant mouse 6Ckine is chemotactic in vitro for thymocytes and activated T cells (Hedrick and Zlotnik). Unlike other C-C chemokines, 6Ckine is not chemotactic for monocytes and neutrophils (Hedrick and Zlotnik, Nagira et al). A growing body of work suggests that 6Ckine influences lymphocyte homing to secondary lymphoid organs (Gunn et al), integrin-mediated lymphocyte adhesion (Pachynski et al) and may act via the EBI1 ligand chemokine (ELC) receptor, CCR7 (Willimann et al, Yoshida et al).
Additional Text
UniProt ID
P84444
Purification
Affinity Purified
Gene ID
100504362; 18829
Gene Name
Ccl21a, Gm1987
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
15
Application Notes
WB: 0.1-0.2 µg/mL. ELISA: 0.5-1.0 µg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX10364 Datasheet
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