Pyruvate Dehydrogenase E1 alpha antibody
Catalogue Number: GTX104015-GTX
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Immunoprecipitation of Pyruvate Dehydrogenase E1 alpha protein from HepG2 whole cell extracts using 5 µg of Pyruvate Dehydrogenase E1 alpha antibody (GTX104015). Western blot analysis was performed using Pyruvate Dehydrogenase E1 alpha antibody (GTX104015). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Pyruvate Dehydrogenase E1 alpha antibody (GTX104015) at 1:300 dilution.
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Immunohistochemical analysis (whole mount) of zebrafish embryo, using Pyruvate Dehydrogenase E1 alpha antibody (GTX104015) at 1:200 dilution.
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Sample (20 µg of whole cell lysate) A: mouse brain 10% SDS PAGE GTX104015 diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Sample (50 µg of whole cell lysate) A: Rat brain 10% SDS PAGE GTX104015 diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Pyruvate Dehydrogenase E1 alpha antibody detects Pyruvate Dehydrogenase E1 alpha protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Pyruvate Dehydrogenase E1 alpha stained by Pyruvate Dehydrogenase E1 alpha antibody (GTX104015) diluted at 1:2000. Blue: Fluoroshield with DAPI (GTX30920).
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Pyruvate Dehydrogenase E1 alpha antibody detects Pyruvate Dehydrogenase E1 alpha protein on whole mount zebrafish by immunohistochemical analysis. Sample: Paraformaldehyde-fixed 1 day-post-fertilization zebrafish embryo. Green: Pyruvate Dehydrogenase E1 alpha stained by Pyruvate Dehydrogenase E1 alpha antibody (GTX104015) diluted at 1:100.
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Various tissue extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Pyruvate Dehydrogenase E1 alpha antibody (GTX104015) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Wild-type (WT) and Pyruvate Dehydrogenase E1 alpha knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Pyruvate Dehydrogenase E1 alpha antibody (GTX104015) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Pyruvate Dehydrogenase E1 alpha antibody detects PDHA1 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. Pyruvate Dehydrogenase E1 alpha antibody (GTX104015) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Pyruvate Dehydrogenase E1 alpha antibody (GTX104015) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 43 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | pyruvate dehydrogenase E1 alpha 1 subunit , PDHA , PDHAD , PDHCE1A , PHE1A |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human Pyruvate Dehydrogenase E1 alpha. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, IP, WB, IHC-WM, IHC-Flt |
Description
Description: The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1); dihydrolipoyl transacetylase (DLAT; MIM 608770) (E2; EC 2.3.1.12); and dihydrolipoyl dehydrogenase (DLD; MIM 238331) (E3; EC 1.8.1.4). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex (Brown et al., 1994 [PubMed 7853374]).[supplied by OMIM]
Additional Text
Gene Name
PDHA1
Gene ID
5160
Uniprot ID
P08559
Purification
Affinity Purified
Concentration
1.39 mg/ml
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
43
Application Notes
WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX104015 Datasheet
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