Glypican 1 antibody [N3C3]
Catalogue Number: GTX104557-GTX
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Glypican 1 antibody [N3C3] (GTX104557) detects Glypican 1 protein by flow cytometry analysis. Sample: A431 cell. Black: Unlabelled sample was used as a control. Red: Glypican 1 antibody [N3C3] (GTX104557) dilution: 1:100. Acquisition of 20,000 events were collected for FACS analysis.
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Immunoprecipitation of Glypican 1 protein from MCF-7 whole cell extracts using 5 µg of Glypican 1 antibody [N3C3] (GTX104557). Western blot analysis was performed using Glypican 1 antibody [N3C3] (GTX104557). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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An ELISA plate is coated with MCF-7 and A431 cells. The coated cells are detected with Glypican 1 antibody (GTX104557) at concentration ranged from 7.5 to 240 ng/mL.
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U87-MG whole cell extract and conditioned medium (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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U87-MG whole cell and membrane extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Mouse tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Untreated (-) and treated (+) A431 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Non-transfected (-) and transfected (+) MCF-7 whole cell extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Untreated (-) and treated (+) MDA-MB-231 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:500 and competitor's antibody (sc-365000) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Untreated (-) and treated (+) A431 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Glypican 1 antibody [N3C3] detects Glypican 1 protein at cell membrane and cytoplasm by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: Glypican 1 stained by Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 µm.
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Glypican 1 antibody [N3C3] detects Glypican 1 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human normal pancreas (left) and pancreatic cancer (right). Glypican 1 stained by Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Glypican 1 antibody [N3C3] detects Glypican 1 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human pancreatic cancer. Glypican 1 stained by Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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The data was published in the journal Nat Commun in 2017. PMID: 28102194
GTX104557 Image
The data was published in the journal Nat Commun in 2017. PMID: 28102194
GTX104557 Image
The data was published in the journal Nat Commun in 2017. PMID: 28102194
GTX104557 Image
The data was published in the journal Nat Commun in 2017. PMID: 28102194
GTX104557 Image
A431 whole cell extracts (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 62 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | glypican 1 , glypican |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human Glypican 1. The exact sequence is proprietary. |
| Application: | ELISA, FC, ICC, IF, IHC-P, IP, WB |
Description
Description: Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. [provided by RefSeq]
Additional Text
Gene Name
GPC1
Gene ID
2817
Uniprot ID
P35052
Concentration
0.71 mg/ml
Purification
Affinity Purified
Molecular Weight
62
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. FCM: 1:50-1:200. IP: 1:100-1:500. ELISA: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX104557 Datasheet
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