PD-L1 antibody
Catalogue Number: GTX104763-GTX
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Untreated (-) and treated (+) MDA-MB-231 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody (GTX104763) diluted at 1:1000.
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Non-transfected (-) and transfected (+) A431 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody (GTX104763) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Various whole cell extracts were separated by 10% SDS-PAGE, and the membranes were blotted with PD-L1 antibody (GTX104763) diluted at 1:600 and with DDDDK tag antibody (GTX115043) diluted at 1:3000 to detect DDDDK-tagged PD-L2. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Untreated (-) and treated (+) A431 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membranes were blotted with PD-L1 antibody (GTX104763) diluted at 1:1200 and competitor's antibody (CST#13684) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.
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PD-L1 antibody detects PD-L1 protein at cell membrane in human ovarian carcinoma by immunohistochemical analysis. Antibodies: PD-L1 antibody (GTX104763) diluted at 1:1000, and competitor's antibody diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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PD-L1 antibody detects PD-L1 protein at cell membrane in PD-L1 protein-expressing cell lines by immunohistochemical analysis. Antibodies: PD-L1 antibody (GTX104763) diluted at 1:1000, and competitor's antibody diluted at 1:50. Samples: Negative (-), low positive (+), intermediate positive (++) and strong positive (+++) cell line cores assessed using Quantitative Digital Pathology. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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PD-L1 antibody detects PD-L1 proteinat cell membrane in human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. PD-L1 antibody (GTX104763) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membranes were blotted with PD-L1 antibody (GTX104763) diluted at 1:2000 and competitor's antibody (CST#13684) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX104763 Image
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody (GTX104763) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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PD-L1 antibody detects PD-L1 protein by immunofluorescent analysis. Sample: MDA-MB-231 (left) and HeLa (right) cells were fixed in ice-cold MeOH for 5 min. Green: PD-L1 stained by PD-L1 antibody (GTX104763) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 µm.
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Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody (GTX104763) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
GTX104763 Image
PD-L1 antibody detects PD-L1 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian cancer. PD-L1 stained by PD-L1 antibody (GTX104763) diluted at 1:4000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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PD-L1 antibody detects PD-L1 protein at cell membrane by immunofluorescent analysis. Sample: MDA-MB-231 cells were fixed in ice-cold MeOH for 5 min. Green: PD-L1 stained by PD-L1 antibody (GTX104763) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
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Wild-type (WT) and PD-L1 knockout (KO) MDA-MB-231 cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody (GTX104763) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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The data was published in the journal Cancers (Basel) in 2019.PMID: 31835799
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The data was published in the journal Cancers (Basel) in 2019.PMID: 31835799
GTX104763 Image
The data was published in the journal Cancers (Basel) in 2019.PMID: 31835799
GTX104763 Image
The data was published in the journal Cancers (Basel) in 2019.PMID: 31835799
GTX104763 Image
The data was published in the journal Cancers (Basel) in 2019.PMID: 31905966
GTX104763 Image
The data was published in the journal Cancers (Basel) in 2019.PMID: 31905966
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The data was published in the 2021 in Cancer Metab. PMID: 33608051
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The data was published in the journal Nat Commun in 2020. PMID: 32245950
GTX104763 Image
The data was published in the journal Nat Commun in 2020. PMID: 32245950
GTX104763 Image
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody (GTX104763) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 33 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | CD274 molecule , B7-H , B7H1 , PD-L1 , PDCD1L1 , PDCD1LG1 , PDL1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide encompassing a sequence within the Intracellular domain of human PD-L1. The exact sequence is proprietary. |
| Application: | FC, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.
Additional Text
Gene Name
CD274
Concentration
0.97 mg/ml
Gene ID
29126
Purification
Affinity Purified
Uniprot ID
Q9NZQ7
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
33
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Short Description
PD-L1 antibody recognizes programmed death-ligand 1 (PD-L1) protein, also known as B7 homolog 1 (B7-H1) and cluster of differentiation 274 (CD274) protein. PD-L1 is found on various cancer cells and transduces immunosuppressive signals by binding to the programmed cell death protein 1 (PD-1) on effector T cells, thereby diminishing immune system attacks on malignant cells. Blocking the interaction between PD-L1 and PD-1 with PD-L1 antibodies is generating great clinical interest as a mode of immunotherapy against a number of cancers.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX104763 Datasheet
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