BMAL1 antibody [N1N3]
Catalogue Number: GTX105060-GTX
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GTX105060 Image
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GTX105060 Image
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Treated 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with BMAL1 antibody [N1N3] (GTX105060) and PER2 antibody (GTX129688) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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BMAL1 antibody [N1N3] detects BMAL1 protein at nucleus by immunofluorescent analysis. Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BMAL1 protein stained by BMAL1 antibody [N1N3] (GTX105060) diluted at 1:500. Blue: Hoechst 33342 staining.
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Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE GTX105060 diluted at 1:1000
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Western Blot analysis of BMAL1 expression in transfected 293T cell line by BMAL1 polyclonal antibody. A: Non-transfected lysate. B: BMAL1 transfected lysate. 7.5% SDS PAGE GTX105060 diluted at 1:500
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Thimerosal|0.01% Thimerosal |
| Molecular Weight: | 69 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | aryl hydrocarbon receptor nuclear translocator like , BMAL1 , BMAL1c , JAP3 , MOP3 , PASD3 , TIC , bHLHe5 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human BMAL1. The exact sequence is proprietary. |
| Application: | ICC, IF, WB |
Description
Description: The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This complex binds an E-box upstream of the PER1 gene, activating this gene and possibly other circadian rhythym-associated genes. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]
Additional Text
Gene ID
406
Gene Name
ARNTL
Uniprot ID
O00327
Concentration
0.65 mg/ml
Purification
Affinity Purified
Molecular Weight
69
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX105060 Datasheet
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