ZEB1 antibody [N2C1], Internal
Catalogue Number: GTX105278-GTX
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GTX105278 Image
GTX105278 Image
GTX105278 Image
GTX105278 Image
GTX105278 Image
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GTX105278 Image
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Cross-linked ChIP was performed with HeLa chromatin extract and 5 µg of either control rabbit IgG or anti-ZEB1 antibody. The precipitated DNA was detected by PCR with primer set targeting to LAMC2 promotor.
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ZEB1 antibody immunoprecipitates ZEB1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 µg 293T whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of ZEB1 protein by 2 µg of ZEB1 antibody (GTX105278) 7.5% SDS-PAGE The immunoprecipitated ZEB1 protein was detected by ZEB1 antibody (GTX105278) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
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Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody (GTX105278) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [N2C1], Internal (GTX105278) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membranes were blotted with ZEB1 antibody [N2C1], Internal (GTX105278) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.
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ZEB1 antibody [N2C1], Internal detects ZEB1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZEB1 protein stained by ZEB1 antibody [N2C1], Internal (GTX105278) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar = 10 µm.
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The data was published in the 2019 in Cancers (Basel). PMID: 31861872
GTX105278 Image
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [N2C1], Internal (GTX105278) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX105278 Image
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [N2C1], Internal (GTX105278) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 124 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | zinc finger E-box binding homeobox 1 , AREB6 , BZP , DELTAEF1 , FECD6 , NIL2A , PPCD3 , TCF8 , ZFHEP , ZFHX1A |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human ZEB1. The exact sequence is proprietary. |
| Application: | ICC, IF, IP, ChIP, WB |
Description
Description: ZEB1 encodes a zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene (MIM 147680) expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site (Williams et al., 1991 [PubMed 1840704]).[supplied by OMIM]
Additional Text
Gene Name
ZEB1
Uniprot ID
P37275
Concentration
0.13 mg/ml
Purification
Affinity Purified
Gene ID
6935
Molecular Weight
124
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX105278 Datasheet
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