GLUD1 + GLUD2 antibody
Catalogue Number: GTX105765-GTX
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GTX105765 Image
GTX105765 Image
GTX105765 Image
GTX105765 Image
GTX105765 Image
GTX105765 Image
GTX105765 Image
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The data was published in the journal Sci Rep in 2018. PMID: 29555943
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GLUD1 + GLUD2 antibody detects Glutamate Dehydrogenase protein at cytoplasm by immunofluorescent analysis. Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Glutamate Dehydrogenase protein stained by GLUD1 + GLUD2 antibody (GTX105765) diluted at 1:500. Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody (GTX631836) diluted at 1:200. Blue: Hoechst 33342 staining.
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Immunohistochemical analysis of paraffin-embedded human breast cancer, using GLUD1 + GLUD2 antibody (GTX105765) antibody at 1:250 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GLUD1 + GLUD2 antibody (GTX105765) diluted at 1:1000.
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GLUD1+ GLUD2 antibody detects Glutamate Dehydrogenase protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Glutamate Dehydrogenase protein stained by GLUD1 + GLUD2 antibody (GTX105765) diluted at 1:500. Blue: Hoechst 33342 staining.
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Drosophila tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with GLUD1 + GLUD2 antibody (GTX105765) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GLUD1 + GLUD2 antibody detects Glutamate Dehydrogenase protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Glutamate Dehydrogenase stained byGLUD1 + GLUD2 antibody (GTX105765) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX105765 Image
GLUD1 + GLUD2 antibody detects Glutamate Dehydrogenase protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. Glutamate Dehydrogenase stained by GLUD1 + GLUD2 antibody (GTX105765) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 61 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | glutamate dehydrogenase 1 , GDH , GDH1 , GLUD |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human Glutamate Dehydrogenase. The exact sequence is proprietary. |
| Application: | ELISA, ICC, IF, IHC-P, WB |
Description
Description: L-glutamate dehydrogenase (EC 1.4.1.3) has a central role in nitrogen metabolism in plants and animals. Glutamate dehydrogenase is found in all organisms and catalyzes the oxidative deamination of 1-glutamate to 2-oxoglutarate (Smith et al., 2001 [PubMed 11254391]). Glutamate, the main substrate of GLUD, is present in brain in concentrations higher than in other organs. In nervous tissue, GLUD appears to function in both the synthesis and the catabolism of glutamate and perhaps in ammonia detoxification (Mavrothalassitis et al., 1988 [PubMed 3368458]).[supplied by OMIM]
Additional Text
Gene Name
GLUD1
Gene ID
2746
Uniprot ID
P00367
Purification
Affinity Purified
Molecular Weight
61
Concentration
0.07 mg/ml
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX105765 Datasheet
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