NDUFS3 antibody
Catalogue Number: GTX105835-GTX
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Wild-type (WT) and NDUFS3 knockout (KO) 293T cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody (GTX105835) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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NDUFS3 antibody detects NDUFS3 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: NDUFS3 stained by NDUFS3 antibody (GTX105835) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10 µm.
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Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody (GTX105835) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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NDUFS3 antibody detects NDUFS3 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. NDUFS3 stained by NDUFS3 antibody (GTX105835) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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NDUFS3 antibody detects NDUFS3 protein at mitochondria by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: NDUFS3 protein stained by NDUFS3 antibody (GTX105835) diluted at 1:500. Blue: Hoechst 33342 staining.
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NDUFS3 antibody detects NDUFS3 protein at cytosol on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. NDUFS3 antibody (GTX105835) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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NDUFS3 antibody detects NDUFS3 protein at cytosol on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. NDUFS3 antibody (GTX105835) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 30 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | NADH:ubiquinone oxidoreductase core subunit S3 , CI-30 , MC1DN8 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human NDUFS3. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB |
Description
Description: This gene encodes one of the iron-sulfur protein (IP) components of mitochondrial NADH:ubiquinone oxidoreductase (complex I). Mutations in this gene are associated with Leigh syndrome resulting from mitochondrial complex I deficiency.
Additional Text
Gene ID
4722
Gene Name
NDUFS3
Uniprot ID
O75489
Concentration
1.31 mg/mL
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
30
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX105835 Datasheet
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