Monoamine Oxidase B antibody [N2C3]
Catalogue Number: GTX105970-GTX
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Monoamine Oxidase B antibody [N2C3] detects Monoamine Oxidase B protein at mitochondria by immunofluorescent analysis. Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: Monoamine Oxidase B stained by Monoamine Oxidase B antibody [N2C3] (GTX105970) diluted at 1:500. Blue: Hoechst 33342 staining.
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Monoamine Oxidase B antibody [N2C3] detects Monoamine Oxidase B protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Monoamine Oxidase B stained by Monoamine Oxidase B antibody [N2C3] (GTX105970) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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ELISA detection of MAOB using GTX105970 for capture at a concentration of 2.5 µg/mL and GTX89368 for detection at a concentration of 0.5 µg/mL.
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The data was published in the journal Int J Mol Sci in 2020.PMID: 32316576
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Monoamine Oxidase B antibody [N2C3] detects Monoamine Oxidase B protein at cytoplasm on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. Monoamine Oxidase B antibody [N2C3] (GTX105970) diluted at 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Monoamine Oxidase B antibody [N2C3] (GTX105970) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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HepG2 whole cell and mitochondria extracts (8.1 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Monoamine Oxidase B antibody [N2C3] (GTX105970) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 59 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | monoamine oxidase B |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human Monoamine Oxidase B. The exact sequence is proprietary. |
| Application: | ELISA, ICC, IF, IHC-P, sELISA, WB, IHC |
Description
Description: The protein encoded by this gene belongs to the flavin monoamine oxidase family. It is a enzyme located in the mitochondrial outer membrane. It catalyzes the oxidative deamination of biogenic and xenobiotic amines and plays an important role in the metabolism of neuroactive and vasoactive amines in the central nervous sysytem and peripheral tissues. This protein preferentially degrades benzylamine and phenylethylamine. [provided by RefSeq]
Additional Text
Gene Name
MAOB
Uniprot ID
P27338
Concentration
0.51 mg/ml
Purification
Affinity Purified
Gene ID
4129
Molecular Weight
59
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. ELISA: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX105970 Datasheet
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