PGK1 antibody
Catalogue Number: GTX107614-GTX
GTX107614 Image
GTX107614 Image
GTX107614 Image
GTX107614 Image
GTX107614 Image
GTX107614 Image
GTX107614 Image
GTX107614 Image
GTX107614 Image
GTX107614 Image
GTX107614 Image
PGK1 antibody detects PGK1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PGK1 protein stained by PGK1 antibody (GTX107614) diluted at 1:20. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000. Blue: Hoechst 33342 staining.
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PGK1 antibody detects PGK1 protein in 1% O2-treated samples by western blot analysis. Upper panel: PGK1 antibody (GTX107614) Lower panel: beta Actin antibody (GTX109639) A. 30 µg HeLa whole cell lysate/extract (untreated) B. 30 µg HeLa whole cell lysate/extract (1% O2 treatment for 24hr) 10% SDS PAGE? PGK1 antibody (GTX107614) dilution: 1:1000 beta Actin antibody (GTX109639) dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Sample (50 µg of whole cell lysate) A: Mouse brain 10% SDS PAGE GTX107614 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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PGK1 antibody detects PGK1 protein by western blot analysis. A. 50 µg Rat liver lysate/extract 10% SDS-PAGE PGK1 antibody (GTX107614) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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PGK1 antibody detects PGK1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody (GTX107614) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody (GTX107614) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody (GTX107614) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Immunofluorescence analysis of methanol-fixed HeLa, using PGK1(GTX107614) antibody at 1:50 dilution.
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The data was published in the journal BMC Genomics in 2015. PMID: 25652794
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Immunohistochemical analysis of paraffin-embedded human hepatoma, using PGK1 antibody(GTX107614) antibody at 1:500 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 45 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | phosphoglycerate kinase 1 , HEL-S-68p , MIG10 , PGKA |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human PGK1. The exact sequence is proprietary. |
| Application: | ELISA, ICC, IF, IHC-P, IP, WB, PLA |
Description
Description: The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. This gene lies on the X-chromosome, while a related pseudogene also has been found on the X-chromosome and another on chromosome 19. [provided by RefSeq]
Additional Text
Gene Name
PGK1
Uniprot ID
P00558
Concentration
0.05 mg/mL
Purification
Affinity Purified
Gene ID
5230
Molecular Weight
45
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:20-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX107614 Datasheet
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