Histone H2A.X antibody
Catalogue Number: GTX108272-GTX
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GTX108272 Image
GTX108272 Image
GTX108272 Image
GTX108272 Image
GTX108272 Image
GTX108272 Image
GTX108272 Image
GTX108272 Image
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GTX108272 Image
GTX108272 Image
GTX108272 Image
GTX108272 Image
GTX108272 Image
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Histone H2A.X antibody detects Histone H2A.X protein at nucleus in human cervical cancer by immunohistochemical analysis. Sample: Paraffin-embedded human cervical cancer. Histone H2A.X antibody (GTX108272) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Histone H2A.X antibody detects Histone H2A.X protein at nucleus in human esophagus cancer by immunohistochemical analysis. Sample: Paraffin-embedded human esophagus cancer. Histone H2A.X antibody (GTX108272) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X antibody (GTX108272) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX108272 Image
Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X antibody (GTX108272) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX108272 Image
Untreated (-) and treated (+) HCT116 whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X antibody (GTX108272) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using Histone H2A.X (GTX108272) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
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Histone H2A.X antibody immunoprecipitates H2AFX protein in IP experiments. IP samples: Jurkat whole cell extract A. 40 µg Jurkat whole cell extract B. Control with 4 µg of preimmune Rabbit IgG C. Immunoprecipitation of H2AFX protein by 4 µg Histone H2A.X antibody (GTX108272) 15 % SDS-PAGE The immunoprecipitated H2AFX protein was detected by Histone H2A.X antibody (GTX108272) diluted at 1:2000. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
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Histone H2A.X antibody detects Histone H2A.X protein by western blot analysis. A. 50 µg mouse brain extract 12% SDS-PAGE Histone H2A.X antibody (GTX108272) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Histone H2A.X antibody detects Histone H2A.X protein by western blot analysis. A. 50 µg rat brain extract 12% SDS-PAGE Histone H2A.X antibody (GTX108272) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Histone H2A.X antibody detects Histone H2A.X protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Histone H2A.X stained by Histone H2A.X antibody (GTX108272) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Histone H2A.X antibody detects Histone H2A.X protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H2A.X stained by Histone H2A.X antibody (GTX108272) diluted at 1:2000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 µm.
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Histone H2A.X antibody detects Histone H2A.X protein at nucleus on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. Histone H2A.X antibody (GTX108272) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
GTX108272 Image
Histone H2A.X antibody detects Histone H2A.X protein at nucleus on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. Histone H2A.X antibody (GTX108272) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
GTX108272 Image
Histone H2A.X antibody detects Histone H2A.X protein at nucleus on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. Histone H2A.X antibody (GTX108272) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
GTX108272 Image
Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X antibody (GTX108272) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 15 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | H2A histone family member X,gamma H2A.X,gamma H2AX,H2A.X,H2A/X,H2AX,H2AFX |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Histone H2A.X. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, IP, WB |
Description
Description: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq]
Additional Text
Gene ID
3014
Uniprot ID
P16104
Concentration
0.19 mg/ml
Purification
Affinity Purified
Gene Name
H2ax
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
15
Application Notes
WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX108272 Datasheet
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