Syntenin 1 antibody [N1], N-term
Catalogue Number: GTX108391-GTX
GTX108391 Image
GTX108391 Image
GTX108391 Image
GTX108391 Image
GTX108391 Image
GTX108391 Image
Immunoprecipitation of Syntenin 1 protein from U87-MG whole cell extracts using 5 µg of Syntenin 1 antibody [N1], N-term (GTX108391). Western blot analysis was performed using Syntenin 1 antibody [N1], N-term (GTX108391). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
GTX108391 Image
Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody [N1], N-term (GTX108391) diluted at 1:500.
GTX108391 Image
HeLa whole cell extracts and HeLa exosome extract (3.5 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody [N1], N-term (GTX108391) diluted at 1:250. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX108391 Image
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Syntenin 1(GTX108391) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
GTX108391 Image
Syntenin 1 antibody [N1], N-term detects SDCBP protein at cytosol on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Syntenin 1 antibody [N1], N-term (GTX108391) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Thimerosal|0.01% Thimerosal |
| Molecular Weight: | 32 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | syndecan binding protein , MDA-9 , MDA9 , ST1 , SYCL , TACIP18 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Syntenin 1. The exact sequence is proprietary. |
| Application: | ELISA, ICC, IF, IHC-P, IP, WB |
Description
Description: The protein encoded by this gene was initially identified as a molecule linking syndecan-mediated signaling to the cytoskeleton. The syntenin protein contains tandemly repeated PDZ domains that bind the cytoplasmic, C-terminal domains of a variety of transmembrane proteins. This protein may also affect cytoskeletal-membrane organization, cell adhesion, protein trafficking, and the activation of transcription factors. The protein is primarily localized to membrane-associated adherens junctions and focal adhesions but is also found at the endoplasmic reticulum and nucleus. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]
Additional Text
Molecular Weight
32
Gene Name
SDCBP
Uniprot ID
O00560
Purification
Affinity Purified
Gene ID
6386
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX108391 Datasheet
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