GFAP antibody
Catalogue Number: GTX108711-GTX
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GFAP antibody detects GFAP protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Green: GFAP antibody (GTX108711) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04). Blue: Nuclear staining with Hoechst 33342. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody (GTX108711) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GFAP antibody detects GFAP protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse hippocampus. Green: GFAP protein stained by GFAP antibody (GTX108711) diluted at 1:250. Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.
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GFAP antibody detects GFAP protein at retinal ganglion cell layer by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: GFAP protein stained by GFAP antibody (GTX108711) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).
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GFAP antibody detects GFAP protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: GFAP protein stained by GFAP antibody (GTX108711) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
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GFAP antibody detects GFAP protein at glia cells by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GFAP stained by GFAP antibody (GTX108711) diluted at 1:500. Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
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GFAP antibody detects GFAP protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse spinal cord. Green: GFAP stained by GFAP antibody (GTX108711) diluted at 1:300. Blue: Hoechst 33342 staining.
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GFAP antibody detects GFAP protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse hippocampus. GFAP stained by GFAP antibody (GTX108711) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX108711 Image
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody (GTX108711) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX108711 Image
GFAP antibody detects GFAP protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. GFAP stained by GFAP antibody (GTX108711) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX108711 Image
GFAP antibody detects GFAP protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. GFAP stained by GFAP antibody (GTX108711) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX108711 Image
Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody (GTX108711) diluted at 1:25000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GFAP antibody detects GFAP protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. GFAP stained by GFAP antibody (GTX108711) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX108711 Image
GFAP antibody detects GFAP protein at glia cells by immunofluorescent analysis. Sample: DIV10 rat E18 primary hippocampal neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GFAP stained by GFAP antibody (GTX108711) diluted at 1:250. Red: Tau, a cytoskeleton marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
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| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 50 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | glial fibrillary acidic protein , ALXDRD |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human GFAP. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB, IHC-Fr, IHC |
Description
Description: This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]
Additional Text
Gene Name
GFAP
Gene ID
2670
Concentration
0.47 mg/ml
Uniprot ID
P14136
Purification
Affinity Purified
Molecular Weight
50
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:5000-1:50000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX108711 Datasheet
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