TrxR1 antibody [N3C2], Internal
Catalogue Number: GTX108727-GTX
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GTX108727 Image
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GTX108727 Image
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TrxR1 antibody [N3C2], Internal detects TrxR1 protein at cytoplasm and nucleus in mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. TrxR1 antibody [N3C2], Internal (GTX108727) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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TrxR1 antibody [N3C2], Internal detects TrxR1 protein at cytoplasm and nucleus in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. TrxR1 antibody [N3C2], Internal (GTX108727) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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TrxR1 antibody [N3C2], Internal detects TXNRD1 protein by western blot analysis. A. 50 µg mouse brain lysate/extract 7.5% SDS-PAGE TrxR1 antibody [N3C2], Internal (GTX108727) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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TrxR1 antibody [N3C2], Internal detects TXNRD1 protein by western blot analysis. A. 50 µg Rat brain lysate/extract 7.5% SDS-PAGE TrxR1 antibody [N3C2], Internal (GTX108727) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TrxR1 (GTX108727) antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using TrxR1 (GTX108727) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Sample (30 µg of whole cell lysate) A: Molt-4 (GTX27912) 7.5% SDS PAGE GTX108727 diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Thimerosal|0.01% Thimerosal |
| Molecular Weight: | 71 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | thioredoxin reductase 1 , GRIM-12 , TR , TR1 , TRXR1 , TXNR |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human TrxR1. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB |
Description
Description: This gene encodes a member of the family of pyridine nucleotide oxidoreductases. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. The functional enzyme is thought to be a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine (Sec) residue which is required for catalytic activity. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenocysteine-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing results in several transcript variants encoding the same or different isoforms. [provided by RefSeq]
Additional Text
Gene Name
TXNRD1
Uniprot ID
Q16881
Purification
Affinity Purified
Gene ID
7296
Molecular Weight
71
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX108727 Datasheet
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