CD146 antibody [C1C3]
Catalogue Number: GTX108777-GTX
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CD146 antibody [C1C3] detects CD146 protein by western blot analysis. A. 50 µg rat liver extract 7.5 % SDS-PAGE CD146 antibody [C1C3] (GTX108777) dilution: 1:1000
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CD146 antibody [C1C3] detects CD146 protein at cell membrane and cytoplasm by immunofluorescent analysis. Sample: HUVEC cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CD146 protein stained by CD146 antibody [C1C3] (GTX108777) diluted at 1:500. Blue: Hoechst 33342 staining.
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Wild-type (WT) and CD146 knockout (KO) HeLa cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD146 antibody [C1C3] (GTX108777) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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CD146 antibody [C1C3] detects CD146 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse placenta. Green: CD146 stained by CD146 antibody [C1C3] (GTX108777) diluted at 1:250. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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CD146 antibody [C1C3] detects CD146 protein by Western blot analysis. A. 30 µg HUVEC whole cell lysate/extract 7.5 % SDS-PAGE CD146 antibody [C1C3] (GTX108777) dilution: 1:10000
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Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD146 antibody [C1C3] (GTX108777) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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The data was published in the journal Stem Cells Transl Med in 2020.PMID: 31943851
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Immunohistochemical analysis of paraffin-embedded J5 xenograft, using CD146(GTX108777) antibody at 1:500 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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CD146 antibody [C1C3] detects CD146 protein at membrane on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. CD146 antibody [C1C3] (GTX108777) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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CD146 antibody [C1C3] detects CD146 protein at membrane on mouse skin by immunohistochemical analysis. Sample: Paraffin-embedded mouse skin. CD146 antibody [C1C3] (GTX108777) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 72 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | melanoma cell adhesion molecule , CD146 , HEMCAM , METCAM , MUC18 , MelCAM |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the C-terminus region of human CD146. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB |
Description
Description: Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration.
Additional Text
Gene Name
MCAM
Concentration
0.21 mg/ml
Purification
Affinity Purified
Gene ID
4162
Uniprot ID
P43121
Molecular Weight
72
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX108777 Datasheet
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