Arginase 1 antibody
Catalogue Number: GTX109242-GTX
GTX109242 Image
GTX109242 Image
GTX109242 Image
GTX109242 Image
GTX109242 Image
GTX109242 Image
GTX109242 Image
GTX109242 Image
GTX109242 Image
GTX109242 Image
GTX109242 Image
GTX109242 Image
GTX109242 Image
GTX109242 Image
GTX109242 Image
GTX109242 Image
GTX109242 Image
GTX109242 Image
GTX109242 Image
Arginase 1 antibody (GTX109242) detects ARG1 protein by flow cytometry analysis. Sample: HepG2 cell. Black: Unlabelled sample was used as a control. Red: Arginase 1 antibody (GTX109242) dilution: 1:50. Acquisition of 20,000 events were collected for FACS analysis.
GTX109242 Image
Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody (GTX109242) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX109242 Image
Immunoprecipitation of Arginase 1 protein from HepG2 whole cell extracts using 5 µg of Arginase 1 antibody (GTX109242). Western blot analysis was performed using Arginase 1 antibody (GTX109242) diluted at 1:600. EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
GTX109242 Image
Sandwich ELISA detection of recombinant full-length Arginase 1 protein using GTX109242 as capture antibody at concentration of 5 µg/mL and GTX634218 as detection antibody at concentration of 1 µg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.
GTX109242 Image
Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634218 as capture antibody at concentration of 5 µg/mL and GTX109242 as detection antibody at concentration of 1 µg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
GTX109242 Image
Arginase 1 antibody detects Arginase 1 protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Arginase 1 stained by Arginase 1 antibody (GTX109242) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10 µm.
GTX109242 Image
Arginase 1 antibody detects Arginase 1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Arginase 1 stained by Arginase 1 antibody (GTX109242) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX109242 Image
Various tissue extracts (50 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody (GTX109242) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX109242 Image
Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody (GTX109242) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX109242 Image
The data was published in the journal Oncotarget in 2016. PMID: 27637084
GTX109242 Image
The data was published in the journal J Neuroinflammation in 2017.PMID: 28196545
GTX109242 Image
The data was published in the journal Sci Rep in 2017.PMID: 28211494
GTX109242 Image
The data was published in the journal PLoS One in 2014.PMID: 24658465
GTX109242 Image
The data was published in the journal Physiol Rep in 2013. PMID: 24303100
GTX109242 Image
The data was published in the journal BMC Microbiol in 2017. PMID: 28835201
GTX109242 Image
The data was published in the journal Oncotarget in 2016. PMID: 27637084
GTX109242 Image
Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody (GTX109242) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
GTX109242 Image
Various tissue extracts (50 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody (GTX109242) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on NCBI mouse tissue.
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 35 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | Arginase 1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Full length human Arginase 1 Recombinant protein. |
| Application: | ELISA, FC, ICC, IF, IHC-P, IP, sELISA, WB, IHC-Fr |
Description
Description: Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. [provided by RefSeq]
Additional Text
Gene Name
ARG1
Gene ID
383
Uniprot ID
P05089
Concentration
0.72 mg/ml
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
35
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. FCM: 1:50-1:200. IP: 1:100-1:1000. ELISA: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX109242 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations