MST1 antibody
Catalogue Number: GTX109294-GTX
GTX109294 Image
GTX109294 Image
GTX109294 Image
GTX109294 Image
GTX109294 Image
GTX109294 Image
STK4 antibody detects STK4 protein at cytoplasm and nucleus in human lung adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung adenocarcinoma. STK4 antibody (GTX109294) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX109294 Image
Wild-type (WT) and MST1 knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with MST1 antibody (GTX109294) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX109294 Image
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using STK4(GTX109294) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
GTX109294 Image
STK4 antibody immunoprecipitates STK4 protein in IP experiments. IP samples: HepG2 whole cell extract A. 50 µg HepG2 whole cell extract B. Control with 4 µg of preimmune Rabbit IgG C. Immunoprecipitation of STK4 protein by 4 µg STK4 antibody (GTX109294) 7.5 % SDS-PAGE The immunoprecipitated STK4 protein was detected by STK4 antibody (GTX109294) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
GTX109294 Image
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using MST1(GTX109294) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Thimerosal|0.01% Thimerosal |
| Molecular Weight: | 56 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | serine/threonine kinase 4 , KRS2 , MST1 , YSK3 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human MST1. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, IP, WB |
Description
Description: The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process. [provided by RefSeq]
Additional Text
Gene ID
6789
Gene Name
Stk4
Purification
Affinity Purified
Molecular Weight
56
Uniprot ID
Q13043
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX109294 Datasheet
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