Cardiac Troponin I antibody [N1C3]
Catalogue Number: GTX109331-GTX
GTX109331 Image
GTX109331 Image
GTX109331 Image
GTX109331 Image
GTX109331 Image
GTX109331 Image
Mouse tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membranes were blotted with Cardiac Troponin I antibody [N1C3] (GTX109331) diluted at 1:3000 and competitor's antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.
GTX109331 Image
Troponin I (cardiac) antibody detects Troponin I (cardiac) protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. Green: Troponin I (cardiac) stained by Troponin I (cardiac) antibody (GTX109331) diluted at 1:250. Red: beta Actin, a cytoskeleton marker, stained by beta Actin antibody [GT5512] (GTX629630) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX109331 Image
Various tissue extracts (50 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cardiac Troponin I antibody [N1C3] (GTX109331) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX109331 Image
Cardiac Troponin I antibody [N1C3] detects Cardiac Troponin I protein at cytosol on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. Cardiac Troponin I antibody [N1C3] (GTX109331) dilution: 1:500. Scale bar = M µm. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
GTX109331 Image
Troponin I (cardiac) antibody [N1C3] detects Troponin I (cardiac) protein at cytosol on rat heart by immunohistochemical analysis. Sample: Paraffin-embedded rat heart. Troponin I (cardiac) antibody [N1C3] (GTX109331) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 24 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | troponin I3, cardiac type , CMD1FF , CMD2A , CMH7 , RCM1 , TNNC1 , cTnI |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Full length human Cardiac Troponin I Recombinant protein. |
| Application: | IHC-P, WB |
Description
Description: Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq]
Additional Text
Gene ID
7137
Gene Name
Tnni3
Uniprot ID
P19429
Molecular Weight
24
Concentration
0.05 mg/mL
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:1000-1:10000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX109331 Datasheet
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