NDUFA12 antibody [N1C3]
Catalogue Number: GTX109567-GTX
GTX109567 Image
GTX109567 Image
GTX109567 Image
GTX109567 Image
GTX109567 Image
GTX109567 Image
NDUFA12 antibody [N1C3] detects NDUFA12 protein at mitochondria in rat colon by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. NDUFA12 antibody [N1C3] (GTX109567) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Mouse tissue extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with NDUFA12 antibody [N1C3] (GTX109567) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX109567 Image
Whole cell extract (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with NDUFA12 antibody [N1C3] (GTX109567) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Immunofluorescence analysis of methanol-fixed HepG2, using NDUFA12(GTX109567) antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NDUFA12(GTX109567) antibody at 1:500 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Thimerosal|0.01% Thimerosal |
| Molecular Weight: | 17 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | NADH:ubiquinone oxidoreductase subunit A12 , B17.2 , DAP13 , MC1DN23 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Full length human NDUFA12 Recombinant protein. |
| Application: | ICC, IF, IHC-P, WB |
Description
Description: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Additional Text
Gene Name
NDUFA12
Uniprot ID
Q9UI09
Gene ID
55967
Concentration
0.89 mg/ml
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
17
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX109567 Datasheet
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