PSMC3 antibody [N1C2]
Catalogue Number: GTX109605-GTX
GTX109605 Image
GTX109605 Image
GTX109605 Image
GTX109605 Image
GTX109605 Image
GTX109605 Image
Immunofluorescent image of a zebrafish embryo eye section using PSMC3 antibody [N1C2] (GTX109605) at a 1:200 dilution. Psmc3 (Green) Actin(Red)(This image was provided courtesy of the Schilling Lab at UC, Irvine.)
GTX109605 Image
Immunofluorescence analysis of paraformaldehyde-fixed A549, using PSMC3(GTX109605) antibody at 1:200 dilution.
GTX109605 Image
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft , using PSMC3(GTX109605) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX109605 Image
Sample (30 ug of whole cell lysate) A: MOLT4 (GTX27912) 10% SDS PAGE GTX109605 diluted at 1:5000
GTX109605 Image
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PSMC3 antibody [N1C2] (GTX109605) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Thimerosal|0.01% Thimerosal |
| Molecular Weight: | 49 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | proteasome 26S subunit, ATPase 3 , TBP1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human PSMC3. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB, IHC-WM |
Description
Description: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases that have chaperone-like activity. This subunit may compete with PSMC2 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. A pseudogene has been identified on chromosome 9. [provided by RefSeq]
Additional Text
Gene ID
5702
Gene Name
PSMC3
Uniprot ID
P17980
Purification
Affinity Purified
Molecular Weight
49
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX109605 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations