Fatty Acid Synthase antibody [N1], N-term
Catalogue Number: GTX109833-GTX
GTX109833 Image
GTX109833 Image
GTX109833 Image
GTX109833 Image
GTX109833 Image
GTX109833 Image
GTX109833 Image
GTX109833 Image
GTX109833 Image
GTX109833 Image
GTX109833 Image
GTX109833 Image
GTX109833 Image
GTX109833 Image
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX109833 Image
Fatty Acid Synthase antibody [N1], N-term detects Fatty Acid Synthase protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Fatty Acid Synthase protein stained by Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:500. Blue: Hoechst 33342 staining.
GTX109833 Image
Fatty Acid Synthase antibody [N1], N-term detects Fatty Acid Synthase protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brown adipocyte. Fatty Acid Synthase stained by Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX109833 Image
FASN antibody immunoprecipitates FASN protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 µg whole cell lysate/extract of FASN protein expressing HeLa cells B : Control with 2.5 µg of pre-immune rabbit IgG C : Immunoprecipitation of FASN by 2.5 µg of FASN antibody (GTX109833) 5% SDS-PAGE The immunoprecipitated FASN protein was detected by FASN antibody (GTX109833) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
GTX109833 Image
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX109833 Image
Fatty Acid Synthase antibody [N1], N-term detects Fatty Acid Synthase protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse white adipocyte. Fatty Acid Synthase stained by Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX109833 Image
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX109833 Image
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX109833 Image
Sample (30 µg of whole cell lysate) A: Molt-4 (GTX27912) 7.5% SDS PAGE GTX109833 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX109833 Image
The data was published in the journal Biomolecules in 2019. PMID: 31614639
GTX109833 Image
Fatty Acid Synthase antibody [N1], N-term detects Fatty Acid Synthase protein at cytoplasm on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
GTX109833 Image
Fatty Acid Synthase antibody [N1], N-term detects Fatty Acid Synthase protein at cytoplasm on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
GTX109833 Image
Immunohistochemical analysis of paraffin-embedded human ovarian carainoma, using Fatty Acid Synthase(GTX109833) antibody at 1:500 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 273 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | fatty acid synthase , FAS , OA-519 , SDR27X1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the N-terminus region of human Fatty Acid Synthase. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, IP, WB, IHC-Fr |
Description
Description: The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq]
Additional Text
Gene Name
FASN
Gene ID
2194
Uniprot ID
P49327
Concentration
0.2 mg/ml
Purification
Affinity Purified
Molecular Weight
273
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX109833 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations