Ku80 antibody [N3C2], Internal
Catalogue Number: GTX109935-GTX
GTX109935 Image
GTX109935 Image
GTX109935 Image
GTX109935 Image
GTX109935 Image
GTX109935 Image
GTX109935 Image
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Ku80 antibody [N3C2], Internal (GTX109935) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX109935 Image
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ku80 antibody [N3C2], Internal (GTX109935) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX109935 Image
Ku80(XRCC5) antibody immunoprecipitates Ku80 protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 µg HeLa whole cell lysate/extract B. Control with 2.5 µg of preimmune rabbit IgG C. Immunoprecipitation of Ku80 protein by 2.5 µg of Ku80 antibody (GTX109935) 7.5% SDS-PAGE The immunoprecipitated Ku80 protein was detected by Ku80 antibody (GTX109935) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
GTX109935 Image
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ku80 antibody [N3C2], Internal (GTX109935) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX109935 Image
Ku80 antibody [N3C2], Internal detects Ku80 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ku80 stained by Ku80 antibody [N3C2], Internal (GTX109935) diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 µm.
GTX109935 Image
Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using Ku80 (XRCC5)(GTX109935) antibody at 1:100 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 83 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | X-ray repair cross complementing 5 , KARP-1 , KARP1 , KU80 , KUB2 , Ku86 , NFIV |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human Ku80. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, IP, WB, PLA |
Description
Description: The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq]
Additional Text
Gene Name
XRCC5
Uniprot ID
P13010
Gene ID
7520
Concentration
0.41 mg/ml
Purification
Affinity Purified
Molecular Weight
83
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX109935 Datasheet
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