O-GlcNAc transferase antibody [N1N2], N-term
Catalogue Number: GTX110136-GTX
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Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with O-GlcNAc transferase antibody [N1N2], N-term (GTX110136) diluted at 1:1000.
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O-GlcNAc transferase antibody [N1N2], N-term detects O-GlcNAc transferase protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: O-GlcNAc transferase stained by O-GlcNAc transferase antibody [N1N2], N-term (GTX110136) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10µm.
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Immunohistochemical analysis (whole mount) of zebrafish embryo, using O-GlcNAc transferase antibody [N1N2], N-term (GTX110136) at 1:200 dilution.
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Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE GTX110136 diluted at 1:1000
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Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE GTX110136 diluted at 1:1000
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O-GlcNAc transferase antibody [N1N2], N-term detects O-GlcNAc transferase protein at cytosol on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. O-GlcNAc transferase antibody [N1N2], N-term (GTX110136) dilution: Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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O-GlcNAc transferase antibody [N1N2], N-term detects OGT protein at cytosol on U373 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U373 xenograft. O-GlcNAc transferase antibody [N1N2], N-term (GTX110136) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Thimerosal|0.01% Thimerosal |
| Molecular Weight: | 117 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | O-linked N-acetylglucosamine (GlcNAc) transferase , HINCUT-1 , HRNT1 , MRX106 , O-GLCNAC , OGT1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the N-terminus region of human O-GlcNAc transferase. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB, IHC-WM |
Description
Description: O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization signal. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]
Additional Text
Gene Name
OGT
Uniprot ID
O15294
Gene ID
8473
Purification
Affinity Purified
Molecular Weight
117
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX110136 Datasheet
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