EZH2 antibody
Catalogue Number: GTX110384-GTX
GTX110384 Image
GTX110384 Image
GTX110384 Image
GTX110384 Image
GTX110384 Image
EZH2 antibody detects EZH2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EZH2 protein stained by EZH2 antibody (GTX110384) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
GTX110384 Image
Cross-linked ChIP was performed with HeLa chromatin extract and 5 µg of either control rabbit IgG or anti-EZH2 antibody. The precipitated DNA was detected by PCR with primer set targeting to CCND2.
GTX110384 Image
Sample (30 µg of whole cell lysate) A: H1299 B: HCT116 C: MCF-7 7.5% SDS PAGE GTX110384 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX110384 Image
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EZH2 antibody (GTX110384) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.01% Thimerosal |
| Molecular Weight: | 85 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | enhancer of zeste 2 polycomb repressive complex 2 subunit , ENX-1 , ENX1 , EZH2b , KMT6 , KMT6A , WVS , WVS2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human EZH2. The exact sequence is proprietary. |
| Application: | ICC, IF, ChIP, WB |
Description
Description: This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]
Additional Text
Gene Name
EZH2
Uniprot ID
Q15910
Gene ID
2146
Concentration
1.42 mg/ml
Purification
Affinity Purified
Molecular Weight
85
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX110384 Datasheet
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