TDG antibody
Catalogue Number: GTX110473-GTX
GTX110473 Image
GTX110473 Image
GTX110473 Image
GTX110473 Image
GTX110473 Image
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TDG antibody (GTX110473) diluted at 1:500.
GTX110473 Image
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TDG antibody (GTX110473) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX110473 Image
TDG antibody detects TDG protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TDG protein stained by TDG antibody (GTX110473) diluted at 1:1000. Blue: Hoechst 33342 staining.
GTX110473 Image
Wild-type (WT) and TDG knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TDG antibody (GTX110473) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Thimerosal|0.01% Thimerosal |
| Molecular Weight: | 46 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | thymine DNA glycosylase , hTDG |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human TDG. The exact sequence is proprietary. |
| Application: | ICC, IF, WB |
Description
Description: The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12. [provided by RefSeq]
Additional Text
Gene Name
TDG
Gene ID
6996
Uniprot ID
Q13569
Purification
Affinity Purified
Molecular Weight
46
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX110473 Datasheet
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