SP1 antibody
Catalogue Number: GTX110593-GTX
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GTX110593 Image
GTX110593 Image
GTX110593 Image
GTX110593 Image
GTX110593 Image
GTX110593 Image
GTX110593 Image
GTX110593 Image
GTX110593 Image
GTX110593 Image
GTX110593 Image
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ChIP was performed with 293T chromatin extract and 5 µg of either normal rabbit IgG or anti-SP1 antibody. The precipitated DNA was detected by PCR with primer set targeting to MGARP promoter.
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Immunoprecipitation of SP1 protein from THP-1 whole cell extracts using 5 µg of OCT1 antibody (GTX110593). Western blot analysis was performed using OCT1 antibody (GTX110593) diluted at 1:250. EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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Non-transfected (-) and transfected (+) 293T whole cell extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody (GTX110593) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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SP1 antibody detects SP1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. SP1 stained by SP1 antibody (GTX110593) diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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SP1 antibody detects SP1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SP1 stained by SP1 antibody (GTX110593) diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
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SP1 antibody detects SP1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. SP1 stained by SP1 antibody (GTX110593) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody (GTX110593) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX110593 Image
SP1 antibody detects SP1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SP1 stained by SP1 antibody (GTX110593) diluted at 1:100. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).
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The data was published in the journal Pharmacol Res Perspect in 2018. PMID: 30455960
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The data was published in the journal Cells in 2020. PMID: 32290085
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The data was published in the 2017 in Oxid Med Cell Longev. PMID: 28740570
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Immunohistochemical analysis of paraffin-embedded Hela xenograft, using SP1(GTX110593) antibody at 1:500 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
GTX110593 Image
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody (GTX110593) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 81 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | Sp1 transcription factor |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human SP1. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, IP, ChIP, WB |
Description
Description: Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression.
Additional Text
Gene Name
SP1
Gene ID
6667
Uniprot ID
P08047
Concentration
0.88 mg/ml
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
81
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX110593 Datasheet
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