Ezrin antibody [N2C2], Internal
Catalogue Number: GTX111709-GTX
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Ezrin antibody [N2C2], Internal detects Ezrin protein at cytoplasm and membrane in rat duodenum by immunohistochemical analysis. Sample: Paraffin-embedded rat duodenum. Ezrin antibody [N2C2], Internal (GTX111709) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Ezrin antibody [N2C2], Internal detects Ezrin protein at cytoplasm by immunofluorescent analysis. Sample: HCT116 cells were fixed in ice-cold MeOH for 5 min. Green: Ezrin protein stained by Ezrin antibody [N2C2], Internal (GTX111709) diluted at 1:1000. Blue: Hoechst 33342 staining.
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Ezrin antibody [N2C2], Internal detects Ezrin protein on whole-mount zebrafish embryos by immunohistochemical analysis. Sample: Paraformaldehyde-fixed zebrafish embryos. Ezrin antibody [N2C2], Internal (GTX111709) dilution: 1:200.
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Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ezrin antibody [N2C2], Internal (GTX111709) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Ezrin antibody [N2C2], Internal detects Ezrin protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissue. Ezrin stained by Ezrin antibody [N2C2], Internal (GTX111709) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Ezrin antibody [N2C2], Internal (GTX111709) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX111709 Image
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Ezrin antibody [N2C2], Internal (GTX111709) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Ezrin antibody [N2C2], Internal detects Ezrin protein at cell membrane by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ezrin stained by Ezrin antibody [N2C2], Internal (GTX111709) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).
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Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using Ezrin(GTX111709) antibody at 1:500 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Ezrin(GTX111709) antibody at 1:500 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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Ezrin antibody [N2C2], Internal detects Ezrin protein on whole mount zebrafish by immunohistochemical analysis. Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo. Green: Ezrin stained by Ezrin antibody [N2C2], Internal (GTX111709) diluted at 1:100. Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70oC
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 69 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | ezrin , CVIL , CVL , HEL-S-105 , VIL2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human Ezrin. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB, IHC-WM |
Description
Description: The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. [provided by RefSeq]
Additional Text
Gene ID
7430
Gene Name
EZR
Uniprot ID
P15311
Concentration
1.84 mg/mL
Purification
Affinity Purified
Molecular Weight
69
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX111709 Datasheet
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