pan Cytokeratin antibody [C-11] (FITC)

Catalogue Number: GTX11212-GTX

Manufacturer:GeneTex
Preservative:15 mM Sodium azide
Physical state:Liquid
Type:Monoclonal Primary Antibody - Conjugated
Shipping Condition:Blue Ice
Unit(s): 100 ul
Host name: Mouse
Clone: C-11
Isotype: IgG1
Immunogen: keratin-enriched preparation from cultured human epidermoid carcinoma cell line A431.
Application: FC, ICC, IF, IHC-P, WB

Description

Description: GT211212 is a broad spectrum antibody which reacts specifically with a wide variety of normal, reactive and neoplastic epithelial tissues. The antibody reacts with simple, cornifying and non-cornifying squamous epithelia and pseudostratified epithelia. It does not react with non-epithelial normal human tissues. This antibody can be applied to methanol- or acetone-fixed frozen sections, and to formalin-fixed, paraffin-embedded human tissues. Increased staining intensity is seen following proteolytic treatment of tested tissue (protease iunmaskingi). Similarly embedded methacarn-fixed material is also suitable for cytokeratin demonstration. Anti-Pan Cytokeratin exhibits a wide interspecies cross-reactivity down to xenopus laevis (e.g., human, bovine, rat, and frog). GT211212 may be used for the localization of cytokeratins using various immunochemical assays including direct immunofluorescence using frozen or fixed tissue sections or cultured cells. It may also be used in flow cytometry of normal and neoplastic epithelial cells. Intermediate-sized filaments are abundant cytoplasmic structural proteins in most vertebrate cells.

Additional Text

Purification

Purified

Antibody Clonality

Monoclonal

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption

Application Notes

ICC/IF: 1:50. IHC-P: 1:50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Storage Note

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles. Protect from light.