alpha Synuclein antibody
Catalogue Number: GTX112799-GTX
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alpha Synuclein antibody detects alpha Synuclein protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Green: alpha Synuclein antibody (GTX112799) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04). Blue: Nuclear staining with Hoechst 33342. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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alpha Synuclein antibody detects alpha Synuclein protein at cytoplasm and nucleus in Rat brain by immunohistochemical analysis. Sample: Paraffin-embedded Rat brain. alpha Synuclein antibody (GTX112799) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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alpha Synuclein antibody detects alpha Synuclein protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: alpha Synuclein protein stained by alpha Synuclein antibody (GTX112799) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).
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alpha Synuclein antibody detects alpha Synuclein protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: alpha Synuclein protein stained by alpha Synuclein antibody (GTX112799) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
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alpha Synuclein antibody detects alpha Synuclein protein by immunofluorescent analysis. Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha Synuclein protein stained by alpha Synuclein antibody (GTX112799) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
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alpha Synuclein antibody detects alpha Synuclein protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse hippocampus. Green: alpha Synuclein protein stained by alpha Synuclein antibody (GTX112799) diluted at 1:250. Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.
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Various tissue extracts (50 µg) were separated by 15% SDS-PAGE, and the membranes were blotted with alpha Synuclein antibody (GTX112799) diluted at 1:2000 and competitor's antibody (MO22112) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.
GTX112799 Image
alpha Synuclein antibody detects alpha Synuclein protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse hippocampus. alpha Synuclein stained by alpha Synuclein antibody (GTX112799) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX112799 Image
Various tissue extracts (50 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with alpha Synuclein antibody (GTX112799) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX112799 Image
alpha Synuclein antibody detects alpha Synuclein protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. alpha Synuclein stained by alpha Synuclein antibody (GTX112799) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX112799 Image
alpha Synuclein antibody detects alpha Synuclein protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. alpha Synuclein stained by alpha Synuclein antibody (GTX112799) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using alpha Synuclein(GTX112799) antibody at 1:250 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with alpha Synuclein antibody (GTX112799) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX112799 Image
Various tissue extracts (50 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with alpha Synuclein antibody (GTX112799) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 14 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | synuclein alpha , NACP , PARK1 , PARK4 , PD1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Full length human alpha Synuclein Recombinant protein. |
| Application: | ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Four alternatively spliced transcripts encoding two different isoforms have been identified for this gene. [provided by RefSeq]
Additional Text
Gene ID
6622
Gene Name
SNCA
Uniprot ID
P37840
Concentration
1.15 mg/ml
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
14
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX112799 Datasheet
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DELIVERY
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