FLI1 antibody
Catalogue Number: GTX112937-GTX
GTX112937 Image
GTX112937 Image
GTX112937 Image
GTX112937 Image
GTX112937 Image
GTX112937 Image
FLI1 antibody detects FLI1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FLI1 protein stained by FLI1 antibody (GTX112937) diluted at 1:500. Blue: Hoechst 33343 staining.
GTX112937 Image
FLI1 antibody immunoprecipitates FLI1 protein in IP experiments. IP samples: Raji whole cell extract A. 40 µg Raji whole cell extract B. Control with 4 µg of preimmune Rabbit IgG C. Immunoprecipitation of FLI1 protein by 4 µg FLI1 antibody (GTX112937) 10 % SDS-PAGE The immunoprecipitated FLI1 protein was detected by FLI1 antibody (GTX112937) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
GTX112937 Image
FLI1 antibody detects FLI1 protein by western blot analysis. A. 50 µg rat kidney lysate/extract 10% SDS-PAGE FLI1 antibody (GTX112937) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX112937 Image
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with FLI1 antibody (GTX112937) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX112937 Image
Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using FLI1(GTX112937) antibody at 1:500 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 51 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | Fli-1 proto-oncogene, ETS transcription factor , BDPLT21 , EWSR2 , SIC-1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human FLI1. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, IP, WB |
Description
Description: Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.
Additional Text
Gene Name
FLI1
Gene ID
2313
Uniprot ID
Q01543
Purification
Affinity Purified
Concentration
1.22 mg/ml
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
51
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX112937 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations