Cytokeratin 8 antibody [N1C1]
Catalogue Number: GTX112975-GTX
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Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 8 antibody [N1C1] (GTX112975) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Sample (50 µg of whole cell lysate) A: mouse liver 10% SDS PAGE GTX112975 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Wild-type (WT) and Krt8 knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Krt8 antibody (GTX112975) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 8 antibody [N1C1] (GTX112975) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Whole zebrafish (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 8 antibody [N1C1] (GTX112975) diluted at 1:700. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cytokeratin 8(GTX112975) antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Cytokeratin 8(GTX112975) antibody at 1:500 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 54 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | keratin 8 , CARD2 , CK-8 , CK8 , CYK8 , K2C8 , K8 , KO |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human Cytokeratin 8. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB |
Description
Description: This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. [provided by RefSeq]
Additional Text
Gene Name
KRT8
Gene ID
3856
Uniprot ID
P05787
Concentration
0.12 mg/ml
Purification
Affinity Purified
Molecular Weight
54
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX112975 Datasheet
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