Arginase 1 antibody
Catalogue Number: GTX113131-GTX
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GTX113131 Image
GTX113131 Image
GTX113131 Image
GTX113131 Image
GTX113131 Image
GTX113131 Image
GTX113131 Image
GTX113131 Image
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Various tissue extracts (50 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody (GTX113131) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Sandwich ELISA detection of recombinant full-length Arginase 1 protein using GTX113131 as capture antibody at concentration of 5 µg/mL and GTX634218 as detection antibody at concentration of 1 µg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.
GTX113131 Image
Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634218 as capture antibody at concentration of 5 µg/mL and GTX113131 as detection antibody at concentration of 1 µg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
GTX113131 Image
Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX113131 as capture antibody at concentration of 5 µg/mL and GTX634218 as detection antibody at concentration of 1 µg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.
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The data was published in the journal Elife in 2017. PMID: 28508748
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The data was published in the journal Neurotherapeutics in 2018.PMID: 30288668
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Arginase 1 antibody detects ARG1 protein at cytosol on Cal27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Cal27 xenograft. Arginase 1 antibody (GTX113131) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
GTX113131 Image
Arginase 1 antibody detects ARG1 protein at cytosol on Cal27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Cal27 xenograft. Arginase 1 antibody (GTX113131) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
GTX113131 Image
Human tissue extract was separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody (GTX113131) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 35 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | Arginase 1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human Arginase 1. The exact sequence is proprietary. |
| Application: | ELISA, ICC, IF, IHC-P, sELISA, WB, IHC |
Description
Description: Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. [provided by RefSeq]
Additional Text
Gene Name
ARG1
Gene ID
383
Uniprot ID
P05089
Concentration
0.41 mg/ml
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
35
Application Notes
WB: 1:5000-1:20000. IHC-P: 1:100-1:1000. ELISA: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX113131 Datasheet
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