Galectin 3 antibody
Catalogue Number: GTX113486-GTX
GTX113486 Image
GTX113486 Image
GTX113486 Image
GTX113486 Image
GTX113486 Image
GTX113486 Image
GTX113486 Image
GTX113486 Image
GTX113486 Image
Wild-type (WT) and Galectin 3 knockout (KO) HeLa cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody (GTX113486) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX113486 Image
Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody (GTX113486) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Sandwich ELISA detection of recombinant full-length Galectin 3 protein using GTX635156 as capture antibody at concentration of 5 µg/mL and GTX113486 as detection antibody at concentration of 1 µg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
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Galectin3 antibody detects Galectin3 protein at cytoplasm by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: Galectin3 protein stained by Galectin3 antibody (GTX113486) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
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Sample (30 ug of whole cell lysate) A: Non-transfected 293T lysates B: LGALS3 transfected 293T lysates 12% SDS PAGE GTX113486 diluted at 1:10000
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Galectin3 antibody detects Galectin3 protein by western blot analysis. A. 50 µg rat colon lysate/extract 12 % SDS-PAGE Galectin3 antibody (GTX113486) dilution: 1:10000
GTX113486 Image
Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody (GTX113486) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using Galectin3(GTX113486) antibody at 1:500 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 26 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | galectin 3 , CBP35 , GAL3 , GALBP , GALIG , L31 , LGALS2 , MAC2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human Galectin 3. The exact sequence is proprietary. |
| Application: | ELISA, ICC, IF, IHC-P, sELISA, WB |
Description
Description: This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. Alternate splicing results in multiple transcript variants.
Additional Text
Gene ID
3958
Gene Name
LGALS3
Uniprot ID
P17931
Concentration
0.85 mg/ml
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
26
Application Notes
WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Sandwich ELISA: . *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX113486 Datasheet
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