CD73 antibody [N1N3]
Catalogue Number: GTX113509-GTX
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GTX113509 Image
GTX113509 Image
GTX113509 Image
GTX113509 Image
GTX113509 Image
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CD73 antibody [N1N3] detects CD73 protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with CD73 antibody [N1N3] (GTX113509) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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CD73 antibody [N1N3] detects CD73 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Green: CD73 stained by CD73 antibody [N1N3] (GTX113509) diluted at 1:250. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Sample (30 µg of whole cell lysate) A: HepG2 B: HCT116 12% SDS PAGE GTX113509 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD73 antibody [N1N3] (GTX113509) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX113509 Image
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD73 antibody [N1N3] (GTX113509) diluted at 1:1000. The HRP-conjated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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Immunohistochemical analysis of paraffin-embedded J5 xenograft, using CD73(GTX113509) antibody at 1:250 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 63 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | 5'-nucleotidase ecto , CALJA , CD73 , E5NT , NT , NT5 , NTE , eN , eNT |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human CD73. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB, NT, Inh, PLA |
Description
Description: Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.[supplied by OMIM]
Additional Text
Gene Name
NT5E
Gene ID
4907
Uniprot ID
P21589
Concentration
0.28 mg/ml
Purification
Affinity Purified
Molecular Weight
63
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX113509 Datasheet
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