ERp57 antibody
Catalogue Number: GTX113719-GTX
GTX113719 Image
GTX113719 Image
GTX113719 Image
GTX113719 Image
GTX113719 Image
GTX113719 Image
GTX113719 Image
GTX113719 Image
GTX113719 Image
ERp57 antibody detects ERp57 protein at endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ERp57 protein stained by ERp57 antibody (GTX113719) diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Hoechst 33342 staining.
GTX113719 Image
ERp57 antibody detects ERp57 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ERp57 antibody (GTX113719) diluted by 1:10000.
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Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERp57 antibody (GTX113719) diluted at 1:500.
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Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membranes were blotted with ERp57 antibody (GTX113719) diluted at 1:5000 and competitor's antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.
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Sample (30 ug of whole cell lysate) A: Neuro2A B: GL261 C: C8D30 7.5% SDS PAGE GTX113719 diluted at 1:1000
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Sample (30 ug of whole cell lysate) A: HepG2 B: HCT116 7.5% SDS PAGE GTX113719 diluted at 1:10000
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ERp57 antibody detects ERp57 protein at cytoplasm on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. ERp57 antibody (GTX113719) diluted at 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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Immunohistochemical analysis of paraffin-embedded U87 xenograft, using ERp57(GTX113719) antibody at 1:500 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Thimerosal|0.01% Thimerosal |
| Molecular Weight: | 57 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | protein disulfide isomerase family A member 3 , ER60 , ERp57 , ERp60 , ERp61 , GRP57 , GRP58 , HEL-S-269 , HEL-S-93n , HsT17083 , P58 , PI-PLC |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human ERp57. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB |
Description
Description: This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. [provided by RefSeq]
Additional Text
Gene Name
PDIA3
Uniprot ID
P30101
Gene ID
2923
Purification
Affinity Purified
Molecular Weight
57
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX113719 Datasheet
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