SDHB antibody
Catalogue Number: GTX113833-GTX
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GTX113833 Image
GTX113833 Image
GTX113833 Image
GTX113833 Image
GTX113833 Image
GTX113833 Image
GTX113833 Image
GTX113833 Image
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SDHB antibody detects SDHB protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in MeOH for 5 min. Green: SDHB protein stained by SDHB antibody (GTX113833) diluted at 1:500. Blue: Hoechst 33342 staining.
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Various tissue extracts (50 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with SDHB antibody (GTX113833) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with SDHB antibody (GTX113833) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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SDHB antibody detects SDHB protein at mitochondria on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. SDHB antibody (GTX113833) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
GTX113833 Image
SDHB antibody detects SDHB protein at mitochondria on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. SDHB antibody (GTX113833) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
GTX113833 Image
SDHB antibody detects SDHB protein at mitochondria on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. SDHB antibody (GTX113833) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
GTX113833 Image
SDHB antibody detects SDHB protein at mitochondria on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. SDHB antibody (GTX113833) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
GTX113833 Image
SDHB antibody detects SDHB protein at mitochondria on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. SDHB antibody (GTX113833) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
GTX113833 Image
SDHB antibody detects SDHB protein at mitochondria on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. SDHB antibody (GTX113833) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 32 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | succinate dehydrogenase complex iron sulfur subunit B , CWS2 , IP , PGL4 , SDH , SDH1 , SDH2 , SDHIP |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human SDHB. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB |
Description
Description: Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis. [provided by RefSeq]
Additional Text
Gene Name
SDHB
Uniprot ID
P21912
Gene ID
6390
Molecular Weight
32
Concentration
0.42 mg/ml
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX113833 Datasheet
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