SNAP25 antibody
Catalogue Number: GTX113839-GTX
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SNAP25 antibody detects SNAP25 protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: SNAP25 protein stained by SNAP25 antibody (GTX113839) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
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SNAP25 antibody detects SNAP25 protein at cell body and synaptic vesicles by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: SNAP25 protein stained by SNAP25 antibody (GTX113839) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
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Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAP25 antibody (GTX113839) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Various tissue extracts (50 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAP25 antibody (GTX113839) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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The data was published in the journal Front Neurol in 2014. PMID: 25002857
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SNAP25 antibody detects SNAP25 protein at cytosol and membrane on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. SNAP25 antibody (GTX113839) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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SNAP25 antibody detects SNAP25 protein at cytosol and membrane on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. SNAP25 antibody (GTX113839) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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SNAP25 antibody detects SNAP25 protein on whole mount zebrafish by immunohistochemical analysis. Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo. Green: SNAP25 stained by SNAP25 antibody (GTX113839) diluted at 1:50. Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70oC
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Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using SNAP25(GTX113839) antibody at 1:100 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 23 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | synaptosome associated protein 25 , CMS18 , RIC-4 , RIC4 , SEC9 , SNAP , SNAP-25 , SUP , bA416N4.2 , dJ1068F16.2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human SNAP25. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB, IHC-Fr, IHC-WM |
Description
Description: Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq]
Additional Text
Gene Name
SNAP25
Uniprot ID
P60880
Gene ID
6616
Concentration
0.27 mg/ml
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
23
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX113839 Datasheet
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